大肠埃希氏菌 ATCC菌种 BIOBW生物

详情描述：

Escherichia coli (Migula) Castellani and Chalmers  拉丁名
(ATCC? 47055?) 统一编号
Classification 类别 Enterobacteriaceae, Escherichia
Strain Designations 菌种别名 S17-1 
Application 用途 transformation host
Biosafety Level 生物安全等级 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 frozen
Storage Conditions	-80℃ or colder
Preceptrol?	no
Genotype	recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 integrated into the chromosome
Type Strain 模式菌株 no
Comments 注释 Recommended mobilization host for pARO180, pARO190, pARO181, and pARO191 (ATCC 77123-77126, respectively) because it contains chromosomally integrated tra genes. A kanamycin-sensitive strain devoid of the E. coli K-12-specific restriction system allowing efficient uptake of foreign DNA. The properties of streptomycin, trimethoprim, and spectinomycin resistance were verified. RP4-Tc::Mu-Km::Tn7 is integrated into the chromosome.
Medium 培养基 ATCC? Medium 1065: LB Agar/Broth, Miller
Growth Conditions 生长条件
Temperature 培养温度: 37℃
Atmosphere 需氧情况 : Aerobic
Genotype	recA pro hsdR RP4-2-Tc::Mu-Km::Tn7 integrated into the chromosome
Name of Depositor 寄存人 D Parke, Department of Biology, Yale University, New Haven, CT
Chain of Custody 来源国家 ATCC <-- D Parke <-- R Simon
References  参考文献 McFarlane GJ, et al. A simplified method for conjugal gene transfer into the filamentous cyanobacterium Anabaena sp. ATCC 27893.
J. Microbiol. Methods 6: 301-305, 1987.
Parke D. Construction of mobilizable vectors derived from plasmids RP4, pUC18 and pUC19. Gene 93: 135-137, 1990. PubMed: 2227423
Simon R, et al. A broad host range mobilization system for in vivo genetic engineering: transposon mutagenesis in Gram negative bacteria. Bio-Technology 1: 784-791, 1983.
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