ATCC菌种菌种购买CBS 6054 树干毕赤酵母

详情描述：

Scheffersomyces stipitis (Pignal) Kurtzman et Suzuki 拉丁名
(ATCC? 58785?) 统一编号
Deposited As	Pichia stipitis Pignal 
Strain Designations 别名 CBS 6054 [CCRC 21777, IFO 10063, NRRL Y-11545] 
Application 用途 Bioethanol production 
Biosafety Level 生物安全等级 1 
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format 提供形式 freeze-dried 
Storage Conditions 保藏条件
Frozen 冷冻物: -80?C or colder
Freeze-Dried 冻干物: 2?C to 8?C 
Live Culture: See Propagation Section 
Type Strain 模式菌种 no 
Preceptrol?	no 
Genome Sequenced Strain	Yes 
Comments 注释 Use of corn steep liquor in fermentation of D-xylose 
Medium 培养基 ATCC? Medium 200: YM agar or YM broth 
ATCC? Medium 1245: YEPD
ATCC? Medium 28: Emmons' modification of Sabouraud's agar 
Growth Conditions 生长条件
Temperature 培养温度: 24?C to 26?C 
Atmosphere 需氧情况: Typical aerobic   
Genome sequencing strain (completed by the Joint Genome Institute at the Department of Energy, USA). Morphology	On YM medium at 25?C after 2 days, colonies creamy white to dingy white, smooth, slightly raised, margin entire. Cells subglobose to ellipsoid, smooth, guttulate, 4-8 X 3-5 μm.
Sequenced Data	18S ribosomal RNA gene, partial sequence; internal transcribed spacer 1, 5.8S ribosomal RNA gene, and internal transcribed spacer 2, complete sequence; and 26S ribosomal RNA gene, partial sequence GGTTTCCGTAGGTGAACCTGCGGAAGGATCATTACAGTATTCTTTTTGCCAGCGCTTAACTGCGCGGCGAAAAAACCTT
ACACACAGTGTTTTCTTTATTAGAAACTATTGCTTTGGTCTGGCTCAGAAATGAGTTGGGCCAGAGGTTTACCAAACTTCA
ATTTTATTGAATTGTTATTTTATTAATTTGTCAATTTGTTGATTAAATTCAAAAATCTTCAAAACTTTCAACAACGGATCTCTT
GGTTCTCGCATCGATGAAGAACGCAGCGAAATGCGATAAGTAATATGAATTGCAGATTTTCGTGAATCATCGAATCTTTGA
ACGCACATTGCGCCCTTTGGTATTCCAAAGGGCATGCCTGTTTGAGCGTCATTTCTCTCTCAAACCCTCGGGTTTGGTATTG
AGTGATACTCTTAGTCGAACTAGGCGTTTGCTTGAAAAGTATTGGCACGAGTGGTACTAAATAGTACTGACAGAATATTTCAATG
TATTAGGTTTATCCAACTCGTTGAGACTTCTGGCGGTGAATTTTTGGTATATTGGCTTTGCCTTACAAAACAACAAACAAGTTTGA
CCTCAAATCAGGTAGGATTACCCGCTGAACTTAAGCATATCAATAA 
D1D2 region of the 26S ribosomal RNA gene ATATCAATAAGCGGAGGAAAAGAAACCAACAGGGATTGCCTTAGTAACGGCGAGTGAAGCG
GCAAAAGCTCAAATTTGAAATCTGGCACCTTCGGTGTCCGAGTTGTAATTTGAAGAAGGTAACTTTGGAGTCAGCTCTTGTC
TATGTTCCTTGGAACAGGACGTCACAGAGGGTGAGAATCCCGTGCGATGAGATGTCTGATTCTATGTA
AAGTGCTTTCGAAGAGTCGAGTTGTTTGGGAATGCAGCTCTAAGTGGGTGGTAAATTCCATCTAAAGCTAAATATTGG
CGAGAGACCGATAGCGAACAAG
TACAGTGATGGAAAGATGAAAAGAACTTTGAAAAGAGAGTGAAAAAGTACGTGAAATTGTTGAAAGGGAAGGGTT
TGAGATCAGACTTGGTATTTTGTATGTCTTGCTTTCGGGTGGGGCCTCTACAGTTTACTGGGCCAGCATCGGTTTGGAC
GGTAGGATAATGACATTGGAATGTGGCACCACTTCGGTGGTGTGTTATAGACTTTGTTGATACTGCCTGTCTAGACCGAGGACTGC
GTCTTTGACTAGGATGCTGGCATAATGATCTTAAACCGC 
Morphology	On YM medium at 25?C after 2 days, colonies creamy white to dingy white, smooth, slightly raised, margin entire. Cells subglobose to ellipsoid, smooth, guttulate, 4-8 X 3-5 μm.
Name of Depositor 寄存者 CBS 
Chain of Custody 来源国家 ATCC <-- CBS <-- J Santa Maria Cross 
References 参考文献 Nucleotide (GenBank) : KU729088 ITS including 5.8S rRNA gene 
Nucleotide (GenBank) : KU729191 D1/D2 region of 28S rRNA gene 
Nucleotide (GenBank) : CM000437 Pichia stipitis CBS 6054 chromosome 1, whole genome shotgun sequence. Nucleotide (GenBank) : AAVQ00000000 Scheffersomyces stipitis CBS 6054 chromosome 1, whole genome shotgun sequencing project Nucleotide (GenBank) : AF030426 nucleotide sequence of PsCYC1 
Nucleotide (GenBank) : U08628 Pichia stipitis CBS 6054 ATCC 58785 autonomous replication 
Nucleotide (GenBank) : U08629 Pichia stipitis CBS 6054 ATCC 58785 orotidine-5'-phosphate 
 Nucleotide (GenBank) : AF127801 Pichia stipitis xylitol dehydrogenase (XYL2) gene, complete cds. 
References	Toivola A, et al. Alcoholic fermentation of D-xylose by yeasts. Appl. Environ. Microbiol. 47: 1221-1223, 1984. 
Deed RW, et al. Xylitol production by recombinant Saccharomyces cerevisiae. Bio-Technology 9: 1090-1095, 1991. PubMed: 1367625 
Amartey S, Jeffries TW. Comparison of corn steep liquor with other nutrients in the fermentation of D-Xylose by Pichia stipitis CBS 6054. Biotechnol. Lett. 16: 211-214, 1994.  
Shi NQ, et al. Disruption of the cytochrome c gene in xylose-utilizing yeast Pichia stipitis leads to higher ethanol production. Yeast 15: 1021-1030, 1999. PubMed: 10455226  
Jeffries TW, et al. Genome sequence of the lignocellulose-bioconvertingand xylose-fermenting yeast Pichia stipitis. Nat. Biotech. 25: 319-326, 2007. PubMed: 17334359 
Wang H, et al. A fungal phylogeny based on 82 complete genomes using the composition vector method. BMC Evol. Biol. 9: 195, 2009. PubMed: 19664262 
Rosenblad MA, et al. Kinship in the SRP RNA family. RNA Biol. 6: 508-516, 2009. PubMed: 19838050 
Taylor DJ, Bruenn J. The evolution of novel fungal genes from non-retroviral RNA viruses. BMC Biol. 7: 88, 2009. PubMed: 20021636 Andersen ES, et al. The tmRDB and SRPDB resources. Nucleic Acids Res. 34: D163-D168, 2006. PubMed: 16381838 
Shi NQ, et al. SHAM-sensitive alternative respiration in the xylose-metabolizing yeast Pichia stipitis. Yeast 19: 1203-1220, 2002. PubMed: 12271457 
Jin YS, et al. Molecular cloning of XYL3 (D-xylulokinase) from Pichia stipitis and characterization of its physiological function. Appl. Environ. Microbiol. 68: 1232-1239, 2002. PubMed: 11872473 
Shi NQ, et al. Characterization and complementation of a Pichia stipitis mutant unable to grow on D-xylose or L-arabinose. Appl. Biochem. Biotechnol. Spring 84-86: 201-216, 2000. PubMed: 10849789